NDT Kidney Art Gallery

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Drug delivery system targeting kidney

From:
Sara Gjeci, Meike N. Leiske, Adrian T. Press
University of Jena and Bayreuth, Germany

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Calcium oxalate monohydrate crystals in a patient with acute kidney injury, many of the dumbbell-shaped type seen under phase contrast and polarized light (Original magnification x400).

From:
Monarch Shah,
Fellow, University of Virginia, USA

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Calcium oxalate monohydrate crystals in a patient with acute kidney injury, many of the dumbbell-shaped type seen under phase contrast and polarized light (Original magnification x400).

From:
Monarch Shah,
Fellow, University of Virginia, USA

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Time projection showing filtration on the surface of a rat kidney acquired using intravital 2-photon microscopy.
A bolus of a small blue dextran appears first in the lumen of the Bowman’s space, proximal tubule lumen, then finally in the cortical collecting duct lumen.
Projection is 300 frames, close to 4 minutes.

From:
Ruben M Sandoval, USA
Indiana Center for Biological Microscopy

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A chronic kidney disease rat model showing the distribution of albumin (red) acquired in 3D using intravital 2-photon microscopy.
Fluorescent cast material (green) can be seen obstructing the lumen of a distal tubule.  The original image was processed to generate a tessellation with seamless borders and natural symmetry.

From:
Ruben M Sandoval, USA
Indiana Center for Biological Microscopy

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Electron microscopy confirmed the electron-dense crystalline structures within cytoplasm of proximal tubular epithelial cells

From:
Hélène Fank, Belgium
MD

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Immunohistochemistry for kappa light chains was strongly positive within these crystals (b, white arrow)

From:
Hélène Fank, Belgium
MD

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Light microscopy (Hematoxylin x400) showing intracellular crystalline inclusions within proximal tubular epithelial cells (a, black arrow)

From:
Hélène Fank, Belgium
MD

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The Greening of Nephrology

From:
Mayleen Laico, Philippines

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Red blood cell cast – brightfield with Sternheimer – Malbin stain

From:
J. Romero Tafoya, México

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Bile cast and hyphae – brightfield with Sternheimer – Malbin stain

From:
J. Romero Tafoya, México

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Red blood cell cast- brightfield with Sternheimer- Malbin stain

From:
J. Romero Tafoya, México

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The Glomerulus ( Not Just Filters)

From:
Anil Saxena, United Arab Emirates
MD, FRCP, FASN Chair,
Nephrology Division Medical Director

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Kidney Transplant
Watercolour painting

From:
Reynaldo Noriega Flores, México

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This image depicts a postnatal mouse kidney immunostained for the renal arterial tree with alpha-Smooth Muscle Actin (SMA, magenta) and glomeruli with podocin (Nphs2, cyan). Kidney was imaged with a LaVision Ultramicroscope II LightSheet using an Olympus MVPLAPO 2x/0.5 objective, a 2x Zoom, Sheet NA of 0.23, a Z step of 2.0µm and image pixel size of 1.54µm/pixel.

From:
Pierre-Emmanuel N’Guetta, USA

Lori O’Brien, USA
PhD, Assistant Professor

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Uric acid crystals, under partial polarization – original magnification x400 – from patient with marked volume depletion and AKI

From:
Jay R. Seltzer, USA
MD, Chief of Nephrology

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Renal tubular epithelial cell cast – brightfield with prolonged Sternheimer-Malbin staining – original magnification x1000 – from patient with bacterial endocarditis, acute kidney injury, and infection related glomerulonephritis

From:
Jay R. Seltzer, USA
MD, Chief of Nephrology

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Uric acid crystals in urine, under fully polarized light – original magnification x400 – from patient with marked volume depletion and AKI

From:
Jay R. Seltzer, USA
MD, Chief of Nephrology

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Red blood cell cast – brightfield with Sternheimer-Malbin stain – original magnification x1000 – from patient with hydralazine-induced vasculitis

From:
Jay R. Seltzer, USA
MD, Chief of Nephrology

NDT Cover July 2023

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This image demonstrates an abstract view of the transition between a proximal tubule and the thick descending limb of the loop of Henle

From:
Tiffany Caza, USA
MD/PhD, a nephropathologist at Arkana Laboratories

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B: Bright field, dark field and phase contrast image of cluster of calcium oxalate dihydrate crystals surrounding leukocytes

From:
Swetha Kanduri, USA
MD, Assistant Professor of Medicine

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Pen and ink sketch

From:
Maia Celeste Arbatin, Philippines
MD FPCP FPSN (#nephgirlsketches)

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Hydrangea Kidney

From:
Maia Celeste Arbatin, Philippines
MD FPCP FPSN (#nephgirlsketches)

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Kidney Trees

From:
Jeanine Verbeke, Belgium

NDT Cover August 2023

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Drawing for a family gift

From:
Dominique Couck, Belgium

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Eyelash sign in glomerular amyloidosis

From:
Boonyarit Cheunsuchon, Thailand
MD, Associate professor

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NDT history

From:
Sonja Djudjaj, Germany
PhD, Assistant Professor

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Water color kidney

From:
Sonja Djudjaj, Germany
PhD, Assistant Professor

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Giving hope

From:
Mayleen Laico, Philippines
MD

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Representative image obtained by STED super-resolution microscopy, showing strong positivity for IgA deposits  (orange) and a partial mild effacement of foot processes (stained with nephrin, cyan) in a biopsy of  IgA nephropathy patient. The biopsy specimen underwent optical tissue clearing before staining.

From:
Maria Lucia Angelotti, Italy
PhD

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Colorized transmission electron microscopy image of murine renal kidney cortex. Individual tubular segments are colored in green, blue and yellow, while a glomerular section is colored in red.

From:
Miriam Buhl, Germany
PhD

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Phase contrast image of cluster of calcium oxalate dihydrate crystals surrounding leukocytes

From:
Swetha Kanduri, USA
MD, Assistant Professor of Medicine

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Bright field image of cluster of calcium oxalate dihydrate crystals surrounding leukocytes

From:
Swetha Kanduri, USA
MD, Assistant Professor of Medicine

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Dark field image of cluster of calcium oxalate dihydrate crystals surrounding leukocytes

From:
Swetha Kanduri, USA
MD, Assistant Professor of Medicine

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Red blood cell cast – brightfield with Sternheimer-Malbin stain – original magnification x1000 – from patient with anti-glomerular basement membrane disease

From:
Jay R. Seltzer, USA
MD, Chief of Nephrology

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Secondary Toni-Debré-FANCONI syndrome – intracellular crystalline inclusions within proximal tubular epithelial cells

From:
Hélène Fank, Belgium
MD

Co-authors:
Antoine Bouquegneau, Christophe Bovy and Orphal Colleye

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Fibrinoid necrosis in a patient with ANCA-associated glomerulonephritis

From:
Boonyarit Cheunsuchon, Thailand
MD, Associate professor